color coding key (zip ties):
pink = hot water
blue = cold water
yellow = poly(i:c)
Warm temp priming
3 aquarium tanks setup with 2 buckets each.
- 1.5 L of water per white bucket (seawater). Don’t put silos in yet (even empty silos) as it can affect bucket water heating
Heating bar and temp gauge in each – heating bar in broader aquarium setup and temp gauge in the bucket.
Set temp on heater manager as desired, wait approx ~1 hr for water to heat, check temp and confirm bucket water temp with gun or glass thermometer.
Want Rphil tank buckets to be at ~34, want cockle buckets at ~30.
Once at temp, can place silos in their respective treatment buckets.
- in treatment buckets for 2h – check temp every 30 min w gun to confirm silo temp
Cold temp priming
6 buckets setup on counter in tank room (no aquarium water bath needed)
1.5 L of water per white bucket (seawater). Don’t put silos in yet
Check and record temp prior to placing silos
Once at temp and warm temp experiment has started, can place silos in their respective treatment buckets.
- in treatment buckets for 2h – check temp every 30 min w gun to confirm silo temp
Poly(i:c) post bath
- put poly(i:c) individuals in one of the yellow tanks post priming for ~30 minutes to allow them to flush the solution out, and then do a water change on that tank
^ June/July experimental logistics timeline
To do / next steps (as of 6/7):
figure out “shelf life” of poly(i:c) stock sol’n
calculate dosage for 1500ml (1.5L)
trial run with cockles and clams this coming week