Preliminary trials to determine what temp manila seed can take for acute priming.
Trial 1
3/17 tested ~30C, ~36C, ~40C with n=6-7 clams each for 2 hours
Seed were held in 12C, 20ppt prior to elevated temp exposure
Water temps measured with infared thermometer
Clams were placed in plastic cups (1 per treatment) with 12C for recovery after exposure
Observations:
30.2C
30C (n=6)
actual water temp upon removal from incubator: 30.2C
Upon removal from incubator (prior to transfer to recovery): 3/6 had slightly opened shells. 3/6 had closed shells
Immediately following transfer to recovery: 6/6 closed shells
5 min following transfer to recovery: 5/6 feet and siphons partially extended, shells slightly open and moving occasionally. 1/6 closed
20 min following transfer to recovery: 6/6 feet and siphons partially extended, shells slightly open and moving occasionally
90 min following transfer to recovery: 6/6 feet and siphons partially extended, shells slightly open, some occasional movement. Responds to contact w dissection probe along mantle (closing shell, retracting foot or siphon)
18 hr: 3/6 slightly open shells with siphons out and responsive to contact with dissection probe. 3/6 shells closed
24 hr: all slightly open and responsive
48 hr: all slightly open and responsive
33.4C
36C (n=6)
actual water temp upon removal from incubator: 33.4C
Upon removal from incubator (prior to transfer to recovery): 3/6 had slightly opened shells. 3/6 had closed shells
Immediately following transfer to recovery: 6/6 closed shells
5 min following transfer to recovery: 6/6 feet and siphons partially extended and moving occasionally, shells slightly open
20 min following transfer to recovery: 6/6 feet and siphons partially extended and moving occasionally, shells slightly open
90 min following transfer to recovery: 6/6 feet and siphons partially extended, shells slightly open, some occasional movement. Responds to contact w dissection probe along mantle (closing shell, retracting foot or siphon)
18 hr: 5/6 slightly open shells with siphons out and responsive to contact with dissection probe. 1/6 shells closed
24 hr: all slightly open and responsive
48 hr: all slightly open and responsive
40.9C
42C (n=7)
actual water temp upon removal from incubator: 40.9C
Upon removal from incubator (prior to transfer to recovery): 6/6 had slightly opened shells with feet and siphons partially extruded
Immediately following transfer to recovery: 6/6 had slightly opened shells with feet and siphons partially extruded, no change from before. When prodded with dissecting probe, no response from any individuals. Indicates 100% mortality
5 min following transfer to recovery: same as previous
20 min following transfer to recovery: same as previous
90 min following transfer to recovery: same as previous. No response to contact w dissection probe along mantle, feet, or siphon
18 hr: same as previous / no change
24 hr: same as previous / no change
48 hr: same as previous / no change
Trial 2
34.5C
36C target temp. 2 hr exposure then moved to 12C for recovery.
actual water temp upon removal from incubator: 34.5C
Upon removal from incubator (prior to transfer to recovery): 2/6 had slightly opened shells with siphons out. 2/6 had closed shells
Immediately following transfer to recovery: 6/6 closed shells
5 min following transfer to recovery: 6/6 feet closed shells
20 min following transfer to recovery: 6/6 feet and siphons partially extended and moving occasionally, shells slightly open and responds to contact w dissection probe along mantle (closing shell, retracting foot or siphon)
18 hr: 6/6 closed shells
24 hr: all slightly open and responsive
48 hr: _____
37.1C
38C target temp. 2 hr exposure then moved to 12C for recovery.
actual water temp upon removal from incubator: 37.1C
Upon removal from incubator (prior to transfer to recovery): 3/6 had slightly opened shells with siphons out. 3/6 had closed shells
Immediately following transfer to recovery: 2/6 open with foot + siphon out, and non responsive to dissection probe contact. 4/6 closed
5 min following transfer to recovery: 2/6 feet open and non responsive. 4/6 closed shells
20 min following transfer to recovery: 5/6 feet and siphons partially extended and moving occasionally, shells slightly open and responds to contact w dissection probe along mantle (closing shell, retracting foot or siphon). 1/6 closed
18 hr: 6/6 closed shells
24 hr: 5/6 slightly open and responsive. 1/6 closed
48 hr: _____